| Marker name | RCS5521 | ||
|---|---|---|---|
| Marker category | Red_clover_SSR | ||
| Primer sequences | Fw | GCTGGAATTGGATACGGAGA | |
| Rv | GTTGACCACAGTACCGCCTT | ||
| EST/Genome sequences | BB913612 | ||
| Lines | |||
| PIC | Value | ||
| Lines | |||
| Map | Woogenellup x Daliak [SNP] | Linkage | LG4 |
| Position | 15239.2 | ||
| Woogenellup x Daliak [SSR] | Linkage | LGd | |
| Position | 184.97 | ||
| Denmark x DGI007 [SSR] | Linkage | LGc | |
| Position | 17.93 | ||
| SNP | Position | ||
| Method | |||
| SSR | Fragment size | 93 | |
| Pattern* | ATC | ||
| Repeat count | 16 | ||
| Method | PCR | TD | |
| Detection | 10% acrylamide | ||
| Multiplex | |||
| Gel image | RCS5521 | ||
| Enzyme | |||
| Related markers | Marker name | RCS5521 | |
| Species | Trifolium pratense | ||
| Comments | Related marker: AP007927, mth2-133o10 ; Related species: Lotus japonicus, Medicago truncatula | ||
* "mis1" and "mis2" in parenthesis indicate imperfect SSR motifs having 1- and 2-base mismatches in the SSR regions, respectively. Perfect SSR motifs have no descriptions.